Vasundhara Dauneria

NuProbe USA Inc.

Sep 2022 - Mar 2023 • 0.5 yrs

Executed end-to-end molecular workflows for ultrasensitive cancer mutation detection platform supporting oncology research and early cancer detection programs. Performed DNA purification, amplification, and NGS library preparation from tissue and cfDNA samples. Executed rare variant analysis workflows targeting gene fusions, copy number alterations (CNAs), and cancer-associated mutations using proprietary BDA™ and QASeq™ technologies. Performed rigorous library quality assessment (fragment size, concentration, adapter ligation) prior to sequencing, reducing failed sequencing runs by 30%. Contributed process improvements reducing repeat runs and waste, directly supporting scalability of cancer genomics workflows Research Focus: Cancer Genomics | Early Cancer Detection | Rare Variant Analysis | cfDNA Analysis | NGS Library Prep Cost Optimization | Data Analysis

Genemed Synthesis Inc.

Oct 2019 - Aug 2022 • 2.8 yrs

Led high-throughput custom antibody production unit delivering 50+ projects annually to global academic, biotech, and pharmaceutical clients across oncology, infectious disease, and renal research programs. Directed end-to-end custom polyclonal antibody development from customer-provided protein sequences through antigen design, carrier conjugation, immunization strategy, purification, and multi- platform validation Oversaw immunization programs in four animal models (rabbit, guinea pig, sheep, goat), optimizing titers and ensuring ethical compliance and reproducibility. Prepared Cyanogen Bromide Sepharose affinity matrices. Supervised team of 4 junior scientists and technicians; implemented standardized SOPs reducing protocol deviations by 40% and improving output quality/turnaround time. Project Impact: Led antibody development for COVID-19 vaccine R&D, supplying validated peptide reagents and antibodies supporting vaccine candidate evaluation Managed oncology biomarker antibody portfolio (5+ projects), supporting preclinical pipelines for checkpoint inhibitors and tumor-associated antigens Directed antibody programs targeting kidney disease markers, contributing critical validation reagents for nephrology research programs Implemented carrier protein activation and conjugation optimization for challenging epitopes, improving immunization success rate by 35%

Alpha Diagnostic International

Nov 2016 - Sep 2019 • 2.8 yrs

Designed and executed precision antibody generation workflows for diagnostic and research applications supporting academic and pharmaceutical clients. Designed antigen strategies from protein sequences using sequence analysis tools (BLAST, CLUSTALW, UniProt), identifying optimal epitopes and improving antibody specificity by 20% Performed carrier protein conjugation (KLH, BSA, OVA) and prepared custom antigens for optimal immunogenicity in rabbit, guinea pig, and sheep models Executed immunization protocols with serial monitoring of antibody titers via ELISA, achieving titers ≥1:50,000 in 85% of projects Conducted downstream antibody purification using affinity chromatography, desalting, dialysis, and size- exclusion chromatography Validated antibody specificity and sensitivity through indirect, sandwich and competitive ELISA, and Western blot assays, achieving >95% specificity Developed and maintained detailed SOPs, experimental records, and validation reports in compliance with ISO standards, supporting regulatory documentation requirements Supported antibody programs used by 15+ major universities and pharmaceutical research groups

Department of Biology, UTSA

Aug 2015 - Aug 2016 • 1 yrs

Delivered laboratory instruction, live experimental demonstrations, and guided problem-solving discussions on enzyme kinetics, protein purification, and metabolic pathways. Designed and maintained course materials including lecture content, laboratory protocols, quizzes, and assessments; updated lab procedures annually Prepared and validated experimental setups and reagents, ensuring consistency and reproducibility across multiple sections per semester Tested laboratory experiments for functionality and created basic usage guides for newly introduced lab equipment.

Department of Biology, UTSA

Sep 2014 - Feb 2015 • 0.4 yrs

Conducted research on toxin-producing cyanobacterial gene regulation and cloning. Led experimental work on luxI gene cloning from the cyanobacterium Microcystis aeruginosa, applying DNA extraction, restriction digestion, and molecular cloning techniques Performed cell and tissue culture-based experiments investigating cyanobacterial quorum sensing mechanisms Refined molecular biology protocols, contributing to laboratory method documentation